1. Optimize codon usage in your gene or cDNA to boost protein expression in heterologous expression systems.
2. Substitute for commonly used cloning methods, such as PCR.
3. Allows the cloning of genes or cDNAs that are difficult to obtain.
4. Synthesize the quantities of cDNA fragments necessary for production of custom microarray gene chips.
5. Clone humanized mouse antibodies or recombinant single chain antibodies.
6. Design genes for gene therapy vectors and DNA vaccines.
7. Engineer genes containing specific mutations, alternative splice variants, single nucleotide polymorphisms, etc.

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